منابع مشابه
Extended Depth of Field for Fluorescence Microscopy
High numerical aperture objectives result in extremely shallow depths of field, which may or may not be desired by the user of the microscope. When looking at extended objects, planes that arent necessarily perpendicular to the light path, or fluorophores that move in and out of focus, an extended depth of field (EDOF) would be useful to fully visualize the sample. PSF engineering can be applie...
متن کاملReal-time extended depth of field microscopy.
We describe an optical microscope system whose focal setting can be changed quickly without moving the objective lens or specimen. Using this system, diffraction limited images can be acquired from a wide range of focal settings without introducing optical aberrations that degrade image quality. We combine this system with a real time Nipkow disc based confocal microscope so as to permit the ac...
متن کاملShaded-Mask Filtering for Extended Depth-of-Field Microscopy
This paper proposes a new spatial filtering approach for increasing the depth-of-field (DOF) of imaging systems, which is very useful for obtaining sharp images for a wide range of axial positions of the object. Many different techniques have been reported to increase the depth of field. However the main advantage in our method is its simplicity, since we propose the use of purely absorbing bea...
متن کاملSimultaneous quantitative depth mapping and extended depth of field for 4D microscopy through PSF engineering
An extended depth of field (EDF) microscope that allows for quantitative axial positioning has been constructed. Past work has shown that EDF microscopy allows for features in varying planes to appear sharply focused simultaneously, however an inherent consequence of this is that depth information is lost. Here, a specifically engineered phase plate is used to create a point spread function (PS...
متن کاملDepth - of - Focus in Microscopy
Digital image processing and analysis provide a powerful tool for describing optical systems. In this paper we show that conventional formulas for the depth-of-focus (∆z) of a high numerical aperture, diffraction-limited optical system are incorrect. Using standard diffraction wave theory we have (rediscovered d a formula for ∆z and then tested that formula under controlled experimental conditi...
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ژورنال
عنوان ژورنال: Optics Express
سال: 2010
ISSN: 1094-4087
DOI: 10.1364/oe.18.003023